河北工程大學(xué)在線發(fā)表《Construction and optimization of a genetic transformation system for efficient expression of human insulin-GFP fusion gene in flax》，文獻(xiàn)中使用了LUYOR-3415RG便攜式雙波長(zhǎng)熒光蛋白激發(fā)光源用于綠色熒光蛋白GFP在亞麻愈傷中的表達(dá)。

文獻(xiàn)摘要如下：

The human insulin gene modified with a C-peptide was synthesized according to the plant-preferred codon, and a fusion gene expression vector of insulin combined with green fluorescent protein (GFP) was constructed. The optimization of the flax callus culturing was undertaken, and a more efficient Agrobacterium-mediated genetic transformation of the flax hypocotyls was achieved. The critical concentration values of hygromycin on the flax hypocotyl development, as well as on its differentiated callus, were explored by the method of antibiotic gradient addition, and the application of antibiotic screening for the verification of positive calluses was assessed. The fusion gene of insulin and GFP was successfully inserted into the flax genome and expressed, as confirmed through polymerase chain reaction and Western blotting. In conclusion, we have established a flax callus culture system suitable for insulin expression. By optimizing the conditions of the flax callus induction, transformation, screening, and verification of a transgenic callus, we have provided an effective way to obtain insulin. Moreover, the herein-employed flax callus culture system could provide a feasible, cheap, and environmentally friendly platform for producing bioactive proteins.

Fluorescence observation of resistant calluses

The transformed explants were observed by a LUYOR-3415RG (Luyang Instrument Co., Ltd, China) excitation light source; the fluorescence was monitored, and the expression of the target protein was assessed.

Flax cells could play high-efficiency chassis roles to express functional foreign protein

When the hypocotyl was 6–8 cm long, we cut it into 0.5 to 1 cm-long pieces. After being infected with Agrobacterium, the hypocotyl was cultured on the callus induction medium. After approximately 2 weeks, some hypocotyls were found to grow calluses. LUYOR-3415RG was used to stimulate the light source to observe the protein expression. After picking out the infected hypocotyls, we continued to observe the growth. It was found that some hypocotyls would turn brown and wither during the induction process, while some other hypocotyls were only partially able to form calluses .

文獻(xiàn)地址：https://link.springer.com/article/10.1186/s40643-024-00799-9   

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LUYOR-3415激發(fā)光源產(chǎn)品介介紹：

LUYOR-3415RG雙波長(zhǎng)熒光蛋白激發(fā)光源