近日，貴州大學(xué)在《iScience ( IF 4.6 )》發(fā)表文獻(xiàn)《Rational Design of 2H-chromene-based Antiphytovirals that Inhibit Virion Assembly by Out-Competing Virus Capsid-RNA Interactions》，文獻(xiàn)中使用路陽儀器上海公司生產(chǎn)的手持式紫外燈LUYOR-3410用于觀察GFP在煙草中的表達(dá)，LUYOR-3410便攜式高強(qiáng)度紫外線燈能夠輸出高強(qiáng)度的紫外線，大面積輻照，被生命科學(xué)研究人員用于uvGFP表達(dá)的觀察，也被材料科學(xué)研究人員用于聚合物材料固化、以及各種化學(xué)材料的紫外輻照。

文獻(xiàn)摘要：

盡管馬鈴薯病毒 Y （PVY） 外殼蛋白 （CP） 的結(jié)構(gòu)基礎(chǔ)的確定為基于 CP 的抗病毒藥物設(shè)計(jì)提供了可能性，但 CP 上的許多特異性殘基在調(diào)節(jié)病毒粒子致病性中的作用在很大程度上是未知的，并且已發(fā)現(xiàn)較少的小分子藥物作用于這些潛在位點(diǎn)。在本研究中，采用分子雜交策略合理設(shè)計(jì)了一系列 2,2-二甲基-2H-鉻合金的衍生物。我們篩選了一例植物病毒殺毒素 C50，它可以與 PVY CP 的 Ser125 形成穩(wěn)定的 H 鍵以發(fā)揮抗病毒特性。Ser125 進(jìn)一步確定對(duì) CP-vRNA 相互作用至關(guān)重要，使 PVY 病毒粒子組裝成為可能。通過與化合物 C50 的競(jìng)爭(zhēng)性結(jié)合，可以顯著抑制這種相互作用。該研究增強(qiáng)了我們對(duì)抗 PVY 藥物機(jī)制的理解，并為開發(fā)新的 CP 靶向病毒顆粒組裝抑制劑提供了基礎(chǔ)。

Although the determination of the structural basis of potato virus Y (PVY) coat protein (CP) provides the possibility for CP-based antiviral drug design, the role of many specific residues on CP in regulating virion pathogenicity is largely unknown, and fewer small-molecular drugs have been discovered to act on these potential sites. In this study, a series of derivatives of 2,2-dimethyl-2H-chromene are rationally designed by employing a molecular hybridization strategy. We screen a case of phytovirucide C50 that could form a stable H-bond with Ser125 of PVY CP to exert antiviral properties. Ser125 is further identified to be crucial for CP-vRNA interaction, enabling PVY virion assembly. This interaction can be significantly inhibited through competitive binding with compound C50. The study enhances our understanding of anti-PVY drug mechanisms and provides a basis for developing new CP-targeting virus particle assembly inhibitors.

Agrobacterium infection of PVY-GFP, agent treatment, and photographing 

The Nicotiana benthamiana plants were subjected to infestation by Agrobacterium tumefaciens, which carried either pCamPVY-GFP or an empty vector (mock). Following this a solution with target compound C50 (500 μg/mL), NNM (500 μg/mL), and a volume equivalent to DMSO (serving as the control) was applied via spraying onto all of the leaves. At dpai, the leaves of N. benthamiana were looked at for the GFP using a LUYOR UV light (High Intensity UV Lamp LUYOR-3410 Shanghai, China), and captured in photographs. The foliage was gathered, rapidly frozen with liquid nitrogen, and subsequently preserved in a refrigerator set at a temperature of -80 ℃. 

文獻(xiàn)全文下載：

PIIS2589004224024350.pdf

路陽儀器上海公司生產(chǎn)有便攜式高強(qiáng)度紫外線燈、臺(tái)式高強(qiáng)度紫外線燈、紫外交聯(lián)儀、紫外透射儀等各種紫外輻照設(shè)備，我們現(xiàn)針對(duì)科研院所終端用戶提供樣機(jī)免費(fèi)試用，有意試用的老師和同學(xué)請(qǐng)網(wǎng)站留言，我們會(huì)及時(shí)和您聯(lián)系。