近日，遼寧省海洋與漁業(yè)科學(xué)研究院在《Marine Science》發(fā)表文獻(xiàn)為《Efficacy of calcein as a chemical marker of Potamocorbula laevis》，文獻(xiàn)中使用了上海路陽(yáng)儀器有限公司銷(xiāo)售的LUYOR-3280LB熒光手電筒用于觀(guān)察鈣黃綠素在海洋生物上的標(biāo)記。鈣黃綠素是一種鈣離子熒光指示劑，激發(fā)波長(zhǎng)為495nm，發(fā)射波長(zhǎng)為515nm。盡管與鈣黃綠素的直接接觸可能會(huì)對(duì)人類(lèi)的皮膚、呼吸道和眼睛造成刺激，但在標(biāo)記生物樣本時(shí)，鈣黃綠素通常通過(guò)內(nèi)部或表面染色方法與細(xì)胞或生物分子結(jié)合，并且已被證明對(duì)人類(lèi)沒(méi)有顯著毒性。LUYOR-3280LB熒光手電筒內(nèi)置了480-490nm藍(lán)光光源，能夠激發(fā)黃綠素發(fā)出黃綠色熒光。

文獻(xiàn)摘要：

Introduction: Calcein was used to develop a shell marking method for Potamocorbula laevis.

Methods: The suitable conditions for marking were investigated, including marking concentration, immersion time, and water temperature. The impacts and feasibility of the marking method were assessed based on the survival rate of P. laevis, the success rate of fluorescence marking, marking quality, and alterations in activities of antioxidant enzymes in the digestive gland of the experimental bivalves. Two concentrations of calcein (20 and 50 mg/L) were used and the immersion time included 1 and 2 h, respectively. The experiment was performed in two rounds, with water temperatures of 12.84 ± 0.09 and 24.18 ± 0.04 °C, respectively.

Results and discussion: The results indicated that calcein did not significantly impact the survival of P. laevis after 7 d of recovery. The catalase activity and malondialdehyde content in low temperature-marked P. laevis showed significant decreases, and the relative abundances of certain fatty acids also exhibited significant changes within 2 h post exposure to 20 mg/L of calcein. However, these indicators returned to normal levels within 7 d. The marking impact of calcein was proportional to the calcein concentration and immersion time. Higher temperature generated a negative impact on the marking effect of 20 mg/L of calcein, while no obvious impacts were observed for 50 mg/L of calcein. The marking success rates and the recapture rates of P. laevis for in situ tests in the two experimental groups were both ** and 4.44 ± 1.29% after one month. Also, the recapture marking rates and the marking good rates of the recaptured individuals were both **. There were no significant differences between these parameters for 50 and 75 mg/L of calcein. Given the cost and safety of labeling, a strategy in terms of an immersion in 50 mg/L of calcein for 2 h could be considered as an effective in situ labeling scheme for P. laevis. In conclusion, calcein can be employed as a marking method for P. laevis. These findings could be potentially beneficial for development of in situ labeling technology, proliferation as well as release of shellfish in tidal flats and resource conservation.

2.5 Grade evaluation of marking effects

After 7 d of marking, all the remaining smooth-shell clams were dissected, and the soft segments were removed. The shells were cleaned and dried at 50°C. Fluorescent markings on the shells were observed using a fluorescence flashlight (Luyor-3280LB) at 480 ~ 490 nm. The quality of fluorescent marking was assessed using Grade 0 ~ 5 (Table 1). Grade 0, 1, 2 and 3 indicated that the marking was not visible, blurry, clearly marking, and brightly marking under the fluorescence flashlight, respectively. Grade 4 and 5 indicated that the marking was visible, and clearly marking under the transmitted light, respectively (Lü et al., 2014). Markings with a quality of no less than Grade 2 were considered acceptable. The success rate of marking and the rate of good markings (Grade 2 or higher) were separately calculated for each experimental group.

文獻(xiàn)地址：https://doi.org/10.3389/fmars.2024.1379571

LUYOR-3280應(yīng)該手電筒產(chǎn)品介紹：

LUYOR-3280RB熒光蛋白觀(guān)察手電筒