近日貴州師范大學(xué)生命科學(xué)學(xué)院在《BMC》期刊發(fā)表文獻(xiàn)《Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry》,文獻(xiàn)中實(shí)驗(yàn)采用LUYOR-3415RG便攜式雙波長(zhǎng)熒光蛋白激發(fā)光源觀察綠色熒光蛋白在毛狀根的表達(dá)。
文獻(xiàn)部分圖片是采用LUV-495A拍照濾鏡和LUYOR-3415RG激發(fā)光源
文獻(xiàn)部分內(nèi)容:
Fluorescence observation of hairy roots
The visualization of GFP fluorescence in transgenic hairy roots was detected using a LUYOR-3415RG dual florescent protein flashlights with a filter cube LUV-495 A 67 mm, excitation wavelength 440–460 nm and emissions wavelength 500 nm (LUYOR, China). All explants, hairy roots, and seedlings were observed using a stereo microscope Olympus SZX16 (Olympus, Japan) equipped with a digital camera Olympus E-330.
文獻(xiàn)下載地址:https://doi.org/10.1186/s13007-023-01078-y
LUYOR-3415RG便攜式雙波長(zhǎng)熒光蛋白激發(fā)光源也叫熒光蛋白觀察手電筒,主要用于觀察各種熒光蛋白在動(dòng)植物體內(nèi)的表達(dá),有365nm,405nm,455nm,505nm,530nm等多種波段可選擇,能夠觀察GFP、EGFP、YFP、BFP、RFP等各種熒光蛋白在植物葉片、愈傷、根系的表達(dá),一級(jí)各種模式動(dòng)物上的表達(dá)。