近期中國熱帶農(nóng)業(yè)科學(xué)院在《Horticultural Plant Journal》期刊上發(fā)表了題為《Agrobacterium tumefaciens-mediated transformation of embryogenic callus and CRISPR/Cas9-mediated genome editing in ‘Feizixiao’ litchi》文獻(xiàn),文獻(xiàn)中采用了LUYOR-3415RG便攜式雙波長激發(fā)光源用于觀察GFP綠色熒光蛋白在荔枝愈傷上的表達(dá)。
LUYOR-3415RG便攜式雙波長激發(fā)光源照射荔枝愈傷,有綠色熒光蛋白的發(fā)出綠色熒光
文獻(xiàn)摘要:
The embryogenic callus was transformed under optimum transformation conditions. After the second round of Hyg screening, resistant yellow callus in 31.58% of the total transformed callus was selected and half was used to induce somatic embryos. Finally, histochemical staining of callus and somatic embryos for GUS gene expression determination and a hand-held lamp (LUYOR 3415RG, Shanghai, China) were used to investigate the expression and resulting fluorescence signals of GFP in resistant transgenic callus and somatic embryos.
文獻(xiàn)地址:https://doi.org/10.1016/j.hpj.2023.01.011
LUYOR-3415RG手持式熒光蛋白激發(fā)光源可檢測轉(zhuǎn)GFP、eGFP、tdtomato、cherry、dsred、dapi、hoechst、cfp、bfp、yfp、venus、lucife等多種熒光蛋白的表達(dá)。激發(fā)光源激發(fā)出綠色熒光時(shí),用黃色濾光鏡觀測,可檢測含綠色熒光蛋白(GFP)的生物;激發(fā)光源激發(fā)紅色熒光時(shí),用紅色濾光鏡觀測,可檢測含紅色熒光蛋白(DsRed)的生物。LUYOR-3415RG雙熒光蛋白激發(fā)光源用于檢測、篩選轉(zhuǎn)綠色熒光蛋白(GFP)和紅色熒光蛋白(DsRed)基因的植物、動(dòng)物及微生物,如水稻、玉米、斑馬魚、小鼠、細(xì)菌、真菌等等, LUYOR-3415RG和LUYOR-3430、LUYOR-3260除了可用于篩選轉(zhuǎn)基因生物外,還可以用于樣本的預(yù)篩選、輔助解剖、用于珊瑚研究等,
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