PMA光解儀用于生物膜存活率檢測

農(nóng)業(yè)農(nóng)村部農(nóng)業(yè)環(huán)境保護(hù)研究所發(fā)表文獻(xiàn)《Revealing the Viable Microbial Community of Biofilm in a Sewage Treatment System Using Propidium Monoazide Combined with Real-Time PCR and Metagenomics》，文獻(xiàn)中的實(shí)驗(yàn)使用了我公司生產(chǎn)的LUYOR-3419PMA光解儀，，使用單疊氮丙啶（PMA）結(jié)合實(shí)時(shí)定量聚合酶鏈?zhǔn)椒磻?yīng)（qPCR）和宏基因組技術(shù)，評估污水處理系統(tǒng)中生物膜的存活率以及存活群落的組成和功能。

文獻(xiàn)摘要：

PMA Condition Optimization

We took 500 μL of viable E. coli suspension and dead E. coli suspension (the concentration was 1 × 108 CFU/mL [47]) and put them into 1.5mL transparent PVC centrifuge tubes, respectively, and then added 500 μL biofilm suspension. The mixtures were thoroughly vortexed. Different volumes of PMA solution (Upland Co., LTD.) were added to achieve final PMA concentrations of 0, 4, 8, 12, 16, and 20 μM. After thorough mixing, the samples were incubated in the dark at room temperature for 5 min and then exposed to light for 10 min using the LUYOR-3419 LED photoreactor (LUYOR INSTRUMENT CO, LTD). The samples were mixed once for 5 min. The LUYOR-3419 LED photoreactor provided uniform and maximal illumination to 24 × 1.5?2 mL vials, using illumination long-lasting LED lights with 465–475 nm emission from both sides and the bottom to all vials for efficient activation of PMA. The distance between the three light sources and vials was 5 mm, with a strength of 7900 μw/cm2 for each vacancy. Subsequently, they were centrifuged at 12,000 rpm for 2 min at 4 °C, and the supernatant was discarded while the sediment was retained for DNA extraction and qPCR detection. Three repetitions were performed in each condition.

文獻(xiàn)地址： https://doi.org/10.3390/microorganisms12081508